By Giovanni Di Sabato, John J. Langone and Helen Van Vunakis (Eds.)
The severely acclaimed laboratory usual, Methods in Enzymology, is among the so much hugely revered courses within the box of biochemistry. considering the fact that 1955, every one quantity has been eagerly awaited, often consulted, and praised through researchers and reviewers alike. The sequence comprises a lot fabric nonetheless suitable this present day - really a necessary ebook for researchers in all fields of existence sciences
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Extra info for Immunochemical Techniques, Part G
E. Billingham, L. Brent, and P. B. Medawar, Proc. R. Sot'. London, Ser. B 143, 58 (1954). METHODS IN ENZYMOLOGY, VOL. 108 Copyright ~t~ 1984 by Academic Press, Inc. All rights of reproduction in any form reserved. 9% sodium chloride) in a small petri dish. If the node does not sink, fat is still attached to it. If necessary, the node can be excised from an anesthetized, living animal by making a smaller incision and suturing the retracted skin together after removing the node. Weighing the N o d e .
All rights of reproduction in any form reserved. 9% sodium chloride) in a small petri dish. If the node does not sink, fat is still attached to it. If necessary, the node can be excised from an anesthetized, living animal by making a smaller incision and suturing the retracted skin together after removing the node. Weighing the N o d e . It is very important to remove excess moisture before weighing. , Whatman No. 50). 01 mg with an analytical balance. A convenient way of doing this is to first weigh an empty piece of weighing paper, then place the nodes one by one on the paper and record the change in weight each time.
15 ml. After the cement dries the chambers are sterilized with ethylene oxide for at least 2 hr. Following sterilization, while still wrapped, they are set aside for 72 hr to lose residual vapors. Thymic cell suspensions, prepared as described above, are injected with a 24-gauge needle into two diffusion chambers. 2 ml of media within the peritoneal cavity of anesthetized animals. The introduction of the cell suspension into the chambers and their implantation into the animals is performed under sterile conditions.