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By Paul M. Vanhoutte, Thomas Gräser, Thomas F. Lüscher (auth.), Gabor M. Rubanyi M.D., Ph.D. (eds.)

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5. GILLESPIE, M. , J. 0. OWASOYO, I. F. McMURTRY, and R. F. O'BRIEN. Sustained coronary vasoconstriction provoked by a peptidergic substance released from endothelial cells in culture. J. Pharmacal. Exp. Ther. 236: 339-343, 1986. 6. HICKEY, K. , G. RUBANYI, R. J. PAUL, and R. F. HIGHSMITH. Characterization of a coronary vasoconstrictor produced by cultured endothelial cells. Am. J. Physiol. 248 (Cell Physiol. 17): C550-C556, 1985. 7. HIGHSMITH, R. , D. C. PANG, and R. M. RAPOPORT. Endothelial cell-derived vasoconstrictors: mechanisms of action in vascular smooth muscle.

Lung Res. 7: 101-112, 1984. 10. HoM, G. , R. F. HIGHSMITH, and D. C. PANG. In vivo hemodynamic effects of endotheliumderived constricting factor in the dog. Drug Dev. Res. 18: 145-151, 1989. 11. O'BRIEN, R. , and I. F. McMURTRY. Endothelial cell supernates contract bovine pulmonary artery rings. Am. Rev. Respir. Dis. 129: 337, 1984. 12. O'BRIEN, R. , R. J. RoBBINS, and I. F. McMuRTRY. Endothelial cells in culture produce a vasoconstrictor substance. J. Cell. Physiol. 132: 263-270, 1987. 13. PANG, D.

H. KURIHARA, S. KIMURA, Y. TOMOBE, M. KOBAYASHI, Y. MITSUI, Y. YAZAKI, K. GOTO, and T. MASAKI. A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature 332: 411-415, 1988. 3 Molecular Biology of Endothelins PENNY E. PHILLIPS, CHRISTINA CADE, LYNNE H. PARKER BOTELHO AND GABOR M. RUBANYI In an attempt to ''bioassay" endothelium-derived relaxing factor (EDRF) produced by cultured bovine aortic endothelial cells, it was discovered by Hickey et al. 7 that the conditioned medium contained a potent vasoconstrictor substance (see Chapter 2).

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