By Zbigniew Darzynkiewicz, J. Paul Robinson, Harry A. Crissman, Leslie Wilson, Paul T. Matsudaira
Each one bankruptcy offers a close history of the defined technique, its theoretical foundations, and its applicability to assorted biomedical fabric. up-to-date chapters describe both the most well-liked equipment or these strategies that experience advanced the main because the previous variation. also, a wide component to the quantity is dedicated to medical cytometry. specific awareness is paid to functions of cytometry in oncology, the main swiftly turning out to be sector. Key positive factors * comprises fifty six wide chapters authored by means of global experts on cytometry* Covers a variety of subject matters, together with rules of cytometry and basic tools, mobile coaching, tandardization and caliber coverage, mobilephone proliferation, apoptosis, cell-cell/cell-environmental interactions, cytogenetics and molecular genetics, telephone functionality and differentiation, experimental and scientific oncology, microorganisms, and infectious illnesses* Describes in-depth the fundamental tools and clinical rules of circulation and laser scanning cytometry and illustrates how they are often utilized to the fields of biology and drugs* enhances the 1st and moment variants on movement cytometry within the equipment in mobile Biology sequence and comprises new sections on know-how ideas.
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Additional resources for Cytometry, Part A
Trujillo, T. , Mullaney, P. , and Coulter, J. R. (1969). Cell microﬂuorometry: A new method for rapid ﬂuorescence measurement. Science 163, 1213–1214. Visser, J. W. , Jongeling, A. A. , and Tanke, H. J. (1979). Intracellular pH determination by ﬂuorescence measurements. J. Histochem. Cytochem. 27, 32–35. Waggoner, A. S. (1990). Fluorescent probes for cytometry. In ‘‘Flow Cytometry and Sorting’’ (M. R. Melamed, T. Lindimo, and M. L. , Chap. 12. Wiley-Liss, New York. Watson, J. U. (1980). Enzyme kinetic studies in cell populations using ﬂuorogenic substrates and ﬂow cytometric techniques.
Science 230, 1401–1403. , and Rukenstroth-Bauer, G. (1979). Flow cytometry as a new method for the measurement of electrophoretic mobility of erythrocytes using membrane charge staining by ﬂuoresceinated polycations. J. Histochem. Cytochem. 27, 342–349. Van Dilla, M. , Trujillo, T. , Mullaney, P. , and Coulter, J. R. (1969). Cell microﬂuorometry: A new method for rapid ﬂuorescence measurement. Science 163, 1213–1214. Visser, J. W. , Jongeling, A. A. , and Tanke, H. J. (1979). Intracellular pH determination by ﬂuorescence measurements.
1979), but it was not available in commercial instruments until many years later. Cell sorting was added to the early ﬂow cytometers for two purposes: (i) diagnostic, that is, for visual identiﬁcation of cells selected by ﬂow cytometry measurement; and (ii) preparative, that is, to collect a pure population of cells based on some selected ﬂow cytometry features. Kamentsky was intent on demonstrating that he had correctly identiﬁed cancer cells in cervical cytology specimens, and he devised a ﬂuidic sorter that brieﬂy diverted the ﬂow into a side channel when a presumed cancer cell was detected (Kamentsky and Melamed, 1967).