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Applying digital pressure to the internal iliac arteries increases the rigidity of the aorta, making collection easier. 5. Verify death by lack of cardiac pulse and fixed, dilated pupils before disposing of carcass. 3) or sedation 1. Restrain the rabbit or sedate with butorphanol/acetylpromazine. 2. Swab the ear with 70% ethanol on a gauze sponge or swab to visualize the vein along the caudal edge of the ear. 5 Current Protocols in Immunology Supplement 73 3. Apply a small amount of d-limonene to the ear to cause vasodilation.

4 Copyright C 2006 by John Wiley & Sons, Inc. 7) 1. Anesthetize the animal. 2a. Decapitate the animal by guillotine (rats) or with a sharp pair of scissors (mice). Allow the animal to hemorrhage via the major cervical vasculature. 2b.

2001). Rats are more docile than mice or hamsters. With training and repeated gentle handling, rats will remain calm with little struggling when restrained (Poole, 1999). Hamsters are excitable animals and will readily bite when surprised. They are nocturnal and tend to sleep during normal laboratory hours. Disturbing a sleeping hamster will readily provoke the animal to bite. Female hamsters, especially pregnant ones, tend to be more aggressive than male hamsters. , 2002). The disposition of most rabbits makes them the easiest of the lab animals to handle (Suckow and Douglas, 1997).

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