By Parakrama Chandrasoma, P. Chandrasoma, Chandrasoma
Univ. of Southern California, l. a.. A Lange scientific e-book. entire synopsis of basic and systemic pathology. For scientific scholars taking their second-year pathology direction. Halftone illustrations. earlier version: c1995. Softcover.
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01% (w/w) Triton X-IOOR. Two main enzyme peaks of NADH-quinone oxidoreductase activity were resolved by this chromatography step; a high-molecular-mass peak (at about 300 kDa; peak I) and a low-molecular-mass peak (at about 90 kDa, peak II). The later one showed about twice as high NADH-duroquinone oxidoreductase activity than the former one. The activity in peak I was strongly inhibited by diphenylene iodonium (DPI). It should be noted that when proteins in the 1OOS fraction had been applied to the size-exclusion column, NADH-quinone oxidoreductase activity 40 was eluted only in fractions corresponding to the position of peak II.
G.. , 1985, 1990 and references therein). In the 1960s it was shown that: (i) erythrocytes reduce the impermeable ferricyanide with concomitant release of protons (Dormandy and Zarday, 1965), and (ii) NADH could be the physiological electron donor in ferricyanide reduction by erythrocyte membranes (Zamudio and Canessa, 1966). The first idea about the presence and putative function of plant PM redox processes appeared many years ago when Lundegardh (1939) and Robertson (1941) proposed a direct association between respiration-linked electron transport and ion uptake in roots.
And Hess, B. ( 1986) Cytochrome b of the Dictyostelium discoideum plasma membrane, Biochim. Biophys. Acta, 852, 315-319. Goldenberg, H. ( 1982) Density gradient fractionation of digitonin-treated rat liver plasma membranes, Enzyme, 27,227-238. , Cordoba, F. and Navas, P. (1998) Involvement of plasma membrane redox systems in growth control of animal cells, in H. Asard, A. J. ), Plasma Membrane Redox Systems and Their Role in Biological Stress and Disease, Kluwer Academic Publishers, Dordrecht, pp.