By Raymond R. Tubbs DO, Mark H. Stoler MD
This quantity within the Foundations in Diagnostic Pathology sequence packs trendy such a lot crucial phone and tissue base molecular pathology right into a compact, high-yield structure! It specializes in the cutting-edge in functional demonstrated molecular diagnostics as utilized around the fields of surgical pathology and cytology. With an emphasis on present, clinically legitimate, and diagnostically vital functions at the present time and within the close to destiny, you may be guaranteed you’re getting the main up to date, authoritative assurance on hand. Its pragmatic, well-organized procedure, approximately 250 full-color illustrations, and at-a-glance bins and tables make the data you wish effortless to entry. useful and reasonable, this source is perfect for research and assessment in addition to daily medical practice!
- Offers designated discussions on today’s applied sciences that can assist you decide on the simplest try out for case evaluate.
- Presents famous molecular pathologists who show the most up-tp-date info, maintaining you at the cusp of your box.
- Features approximately 250 full-color illustrations that current vital pathologic positive aspects, allowing you to shape a differential analysis and evaluate your findings with genuine situations.
- Uses a constant, basic layout, together with at-a-glance packing containers and tables for simple reference.
The Foundations in Diagnostic Pathology sequence answers the decision for clean, cheap, and easy-to-use advice. each one region-specific quantity offers the entire such a lot crucial details at the pathologic entities encountered in perform. sequence Editor: John R. Goldblum, MD, FACP, FASCP, FACG
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Extra info for Cell and Tissue Based Molecular Pathology
J Mol Endocrinol 2000;25:169–193. DiDomenico N, Link H, Knobel R, et al: Cobas Amplicor™: Fully automated RNA and DNA ampliﬁcation and detection system for routine diagnostic PCR. Clin Chem 1996;42:1915–1923. du Manoir S, Speicher MR, Joos S, et al: Detection of complete and partial chromosome gains and losses by comparative genomic in situ hybridization. Hum Genet 1993;90:590–610. Dyanov HM, Dzitoeva SG: Method for attachment of microscopic preparations on glass for in situ hybridization, PRINS, and in situ PCR studies.
Du Manoir S, Speicher MR, Joos S, et al: Detection of complete and partial chromosome gains and losses by comparative genomic in situ hybridization. Hum Genet 1993;90:590–610. Dyanov HM, Dzitoeva SG: Method for attachment of microscopic preparations on glass for in situ hybridization, PRINS, and in situ PCR studies. BioTechniques 1995;18:823–826. Esch RK: Basic nucleic acid procedures. In Coleman WB, Tsongalis GJ (eds): Molecular Diagnostics for the Clinical Laboratorian. Totowa, NJ, Humana Press, 1997, pp 55–58.
There is a developing interest in obtaining “free” nucleic acid as a means to detect or monitor neoplastic disease. Finally, DNA that has been excised from a host genome and cloned into vector DNA molecules such as plasmids or cosmids is often used as positive control material in PCR ampliﬁcation. RNA To detect or quantify RNA transcripts or the RNA genomes of some viruses, RNA must be converted into DNA so that it can serve as a template in a PCR ampliﬁcation. This process is called reverse transcription (RT) and harnesses enzymes from retroviruses that are used to integrate their RNA genomes into host genomic DNA.